Suction Blister Model of Wound Healing
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The suction blister model was originally developed for the separation of viable epidermis from dermis by Kiistala (1). Since its development, its use has been expanded and applied to several other applications. One new application assesses the collagen synthesis rate in the human skin in vivo by using the suction blister fluid collected in an assay of collagen propeptides (2). This method is sensitive and can detect changes in collagen synthesis owing to various diseases and topical or systemic therapies. Other applications of the suction blister technique include measurements of pharmacological agents or their derivatives from interstitial fluid, and assays of various enzymes, cytokines, and so on (3-7). Additionally, the suction blister technique has even been used to treat vitiligo by collecting living melanocytes from blister roofs from healthy skin and injecting these into blister cavities induced in diseased skin.
- Kiistala, U. (1968) Suction blister device for separating viable epidermis from dermis. J. Invest. Dermatol. 50, 129–137.
- Oikarinen, A., Autio, P., Kiistala, U., Risteli, L., and Risteli, J. (1992) A new method to measure type I and III collagen synthesis in human skin in vivo: demonstration of decreased collagen synthesis after topical glucocorticoid treatment. J. Invest. Dermatol. 98, 220–225. CrossRef
- Ingemansson-Nordqvist, B., Kiistala, U., and Rorsman, H. (1967) Culture of adult human epidermal cells obtained from roofs of suction blisters. Acta Dermatol. Venereol. 47, 237–240.
- Averbeck, D., Averbeck, S., Blais, J., Moysan, A., Hüppe, G., Morliére, P., Prognon, P., Vigny, P., and Dubertret, L. (1989) Suction blister fluid: its use for pharmacodynamic and toxicological studies of drugs and metabolites in vivo in human skin after topical or systemic administration, in Models in Dermatology, vol. 4 (Maibach, H. I. and Lowe, N. J., eds.), Karger, Basel, pp. 5–11.
- Benfeldt, E., Serup, J., and Menné, T. (1999) Microdialysis vs. suction blister technique for in vivo sampling of pharmacokinetics in the human dermis. Acta Dermatol. Venereol. 79, 338–342. CrossRef
- Oikarinen, A., Kylmäniemi, M., Autio-Harmainen, H., Autio, P., and Salo, T. (1993) Demonstration of 72-kDa and 92-kDa forms of type IV collagenase in human skin: variable expression in various blistering diseases, induction during re-epithelialization and decrease by topical glucocorticoids. J. Invest. Dermatol. 101, 205–210. CrossRef
- Kylmäniemi, M., Autio, P., and Oikarinen, A. (1994) Interleukin 1alfa (IL-1alfa) in human skin in vivo. Lack of correlation to markers of collagen metabolism. Acta Dermatol. Venereol. 74, 364–367.
- Kiistala, U. and Mustakallio, K. K. (1967) Dermo-epidermal separation with suction: electron microscopic and histochemical study of initial events of blistering on human skin. J. Invest. Dermatol. 48, 466–477.
- Oikarinen, A., Savolainen, E.-R., Tryggvason, K., Foidart, J.-M., and Kiistala, U. (1982) Basement membrane components and galactosylhydroxylysyl glucosyltransferase in suction blisters of human skin. Br. J. Dermatol. 106, 257–266. CrossRef
- Kainulainen, T., Häkkinen, L., Hamidi, S., Larjava, K., Kallioinen, M., Peltonen J., Salo, T., Larjava, H., and Oikarinen, A. (1998) Laminin-5 expression is independent of the injury and microenvironment during re-epithelialization of wounds. J. Histochem. Cytochem. 46, 353–360. CrossRef
- Leivo, T., Kiistala, U., Vesterinen, M., Owaribe, K., Burgeson, R. E., Virtanen, I., and Oikarinen, A. (2000) Re-epithelialization rate and protein expression in suction-induced wound model: comparison between intact blisters, open wounds and calcipotrial-pretreated open wounds. Br. J. Dermatol. 142, 991–1002. CrossRef
- Kiistala, U. (1972) Dermal-epidermal separation II. External factors in suction blister formation with special reference to the effects of temperature. Ann. Clin. Res. 4, 236–246.
- Koivukangas, V., Annala, A.-P., Salmela, P. I., and Oikarinen, A. (1999) Delayed restoration of epidermal barrier function after suction blister injury in patients with diabetes mellitus. Diabet. Med. 16, 563–567. CrossRef
- Koivukangas, V. and Oikarinen, A. (1998) Effects of PUVA and UVB treatment on restoration of epidermal barrier function and vascular response after suction blister injury in human skin in vivo. Photodermatol. Photoimmunol. Photomed. 14, 119–124. CrossRef
- Suction Blister Model of Wound Healing
- Book Title
- Wound Healing
- Book Subtitle
- Methods and Protocols
- Book Part
- pp 255-261
- Print ISBN
- Online ISBN
- Series Title
- Methods in Molecular Medicine™
- Series Volume
- Series ISSN
- Humana Press
- Copyright Holder
- Humana Press
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