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E. coli pp 197–208Cite as

Methods for the Identification of Host Receptors for Shiga Toxin

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Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 73))

Abstract

The Shiga toxin receptor glycolipid Gb3 (globotriaosylceramide) is also known as the pk blood group antigen (1) and as CD77, a germinal center B-cell differentiation antigen (2). Tissue localization of Gb3 is associated with sites of Stx-induced pathology in animal models (3–5) and in humans (6) and, as such, is a major determinant of Stx1-induced disease.

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References

  1. Naiki M. and Marcus D. M. (1974) Human erythrocyte P and Pk blood group antigens: identification as glycosphingolipids. Biochem. Biophys. Res. Commun. 60, 1105–1111.

    Article  CAS  PubMed  Google Scholar 

  2. Mangeney M., Richard Y., Coulaud D., Tursz T., and Wiels J. (1991) CD77: an antigen of germinal center B cells entering apoptosis. Eur. J. Immunol. 21, 1131–1140.

    Article  CAS  PubMed  Google Scholar 

  3. Boyd B., Tyrrell G., Maloney M., Gyles C., Brunton J., and Lingwood C. (1993) Alteration of the glycolipid binding specificity of the pig edema toxin from globotetraosyl to globotriaosyl ceramide alters in vivo tissue targetting and results in a Stx1-like disease in pigs. J. Exp. Med. 177, 1745–1753.

    Article  CAS  PubMed  Google Scholar 

  4. Hertzke D. M., Cowan L. A., Schoning P., and Fenwick B. W. (1995) Glomerular ultrastructural lesions of idiopathic cutaneous and renal glomerular vasculopathy of greyhounds. Vet. Pathol. 32, 451–459.

    Article  CAS  PubMed  Google Scholar 

  5. Zoja J., Corna D., Farina C., Sacchi G., Lingwood C., Doyles M., et al. (1992) Verotoxin glycolipid receptors determine the localization of microangiopathic process in rabbits challenged with verotoxin-1. J. Lab. Clin. Med. 120, 229–238.

    CAS  PubMed  Google Scholar 

  6. Lingwood C. A. (1994) Verotoxin-binding in human renal sections. Nephron 66, 21–28.

    Article  CAS  PubMed  Google Scholar 

  7. Katagiri Y., Mori T., Nakajima H., Katagiri C., Taguchi T., Takeda T., et al.(1999) Activation of Src family kinase induced by Shiga toxin binding to globotriaosyl ceramide (Gb3/CD77) in low density, detergent-insoluble microdomains. J. Biol. Chem. 274, 35,278–35,282.

    Article  CAS  PubMed  Google Scholar 

  8. Cohen A., Hannigan G. E., Williams B. R. G., and Lingwood C. A. (1987) Roles of globotriosyl-and galabiosylceramide in verotoxin binding and high affinity interferon receptor. J. Biol. Chem. 262, 17,088–17,099.

    CAS  PubMed  Google Scholar 

  9. Ghislain J., Lingwood C. A., and Fish E. N. (1994) Evidence for glycosphingolipid modification of the type 1 IFN receptor. J. Immunol. 153, 3655–3663.

    CAS  PubMed  Google Scholar 

  10. Khine A. A. and Lingwood C. A. (2000) Functional significance of globotriaosylceramide in α2 interferon/ type I interferon receptor mediated antiviral activity. J. Cell. Physiol. 182, 97–108.

    Article  CAS  PubMed  Google Scholar 

  11. van Setten P., Monnens L., Verstraten R., van der Heuvel L. and van Hinsberg V. (1996) Effects of verotoxin-1 on non adherent human monocytes: binding characteristics, protein synthesis, and induction of cytokine release. Blood 88, 174–183.

    PubMed  Google Scholar 

  12. Ramegowda B. and Tesh V. L. (1996) Differentiation-associated toxin receptor modulation, cytokine production, and sensitivity to Shiga-like toxins in human monocytes and monocytic cell lines. Infect. Immun. 64, 1173–1180.

    CAS  PubMed  Google Scholar 

  13. Sakiri R., Ramegowa B., and Tesh V. L. (1998) Shiga toxin type 1 activates tumor necrosis factor-α gene transcription and nuclear translocation of the transcriptional activators nuclear factor-kB and activator protein-1. Blood 92, 558–566.

    CAS  PubMed  Google Scholar 

  14. Arab S., Russel E., Chapman W., Rosen B. and Lingwood C. (1997) Expression of the Verotoxin receptor glycolipid, globotriaosylceramide, in ovarian hyperplasias. Oncol. Res. 9, 553–563.

    CAS  PubMed  Google Scholar 

  15. LaCasse E. C., Bray M. R., Patterson B., Lim W.-M., Perampalam S., Radvanyi L. G., et al. (1999) Shiga-like toxin I receptor on human breast cancer, lymphoma, and myeloma and absence from CD34+ hematopoietic stem cells: implications for ex vivo tumor purging and autologous stem cell transplantation. Blood 94, 1–12.

    Google Scholar 

  16. Keusch J., Manzella S. M., Nyame K. A., Cummings R. D. and Baenziger J. U.(2000) Cloning of Gb3 synthase, the key enzyme in globo-series glycosphingolipid synthesis, predicts a family of of α1,4 glycosyltransferases conserved in plants, insects and mammals. J. Biol. Chem. 275, 25,315–25,321.

    Article  CAS  PubMed  Google Scholar 

  17. Sandvig K., Garred Ø., Prydz K., Kozlov J., Hansen S., and van Deurs B.(1992) Retrograde transport of endocytosed Shiga toxin to the endoplasmic reticulum. Nature 358, 510–512.

    Article  CAS  PubMed  Google Scholar 

  18. Arab S. and Lingwood C. (1998) Intracellular targeting of the endoplasmic reticulum/nuclear envelope by retrograde transport may determine cell hypersensitivity to Verotoxin: sodium butyrate or selection of drug resistance may induce nuclear toxin targeting via globotriosyl ceramide fatty acid isoform traffic. J. Cell. Physiol. 177, 646–660.

    Article  CAS  PubMed  Google Scholar 

  19. Kasai K., Galton J., Terasaki P., Wakisaka A., Kawahara M., Root T., et al. (1985) Tissue distribution of the Pk antigen as determined by a monoclonal antibody. J. Immunogenet. 12, 213–220.

    Article  CAS  PubMed  Google Scholar 

  20. Pallesen G. and Zeuthen J. (1987) Distribution of the Burkitt’s-lymphoma-associated antigen (BLA) in normal human tissue and malignant lymphoma as defined by immunohistological staining with monoclonal antibody 38.13. J. Cancer Res. Clin. Oncol. 113, 78–86.

    Article  CAS  PubMed  Google Scholar 

  21. Oosterwijk E., Kalisiak A., Wakka J., Scheinberg D., and Old L. J. (1991) Monoclonal antibodies against Galα1-4Galß1-4Glc (Pk, Cd77) produced with a synthetic glycoconjugate as immunogen: reactivity with carbohydrates, with fresh frozen human tissues and hematopoietic tumors. Int. J. Cancer 48, 848–854.

    Article  CAS  PubMed  Google Scholar 

  22. Kiarash A., Boyd B., and Lingwood C. A. (1994) Glycosphingolipid receptor function is modified by fatty acid content: Verotoxin 1 and Verotoxin 2c preferentially recognize different globotriaosyl ceramide fatty acid homologues. J. Biol. Chem. 269, 11,138–11,146.

    CAS  PubMed  Google Scholar 

  23. Yiu S. C. K. and Lingwood C. A. (1992) Polyisobutylmethacrylate modifies glycolipid binding specificity of verotoxin 1 in thin layer chromatogram overlay procedures. Anal. Biochem. 202, 188–192.

    Article  CAS  PubMed  Google Scholar 

  24. Folch J., Lees M., and Sloane-Stanley G. H. (1957) A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226, 497–521.

    CAS  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. Departments of Laboratory Medicine and Pathobiology and Biochemistry, University of Toronto and The Research Institute, Hospital for Sick Children, Toronto, Canada

    Anita Nutikka, Beth Binnington-Boyd & Clifford A. Lingwood

Authors
  1. Anita Nutikka
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  2. Beth Binnington-Boyd
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  3. Clifford A. Lingwood
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Editor information

Editors and Affiliations

  1. Groupe d&'Immunité Innée et Signalisation, Institut Pasteur, Paris, France

    Dana Philpott

  2. Max-von-Pettenkofer-Institut, Bakteriologie, Munich, Germany

    Frank Ebel

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© 2003 Humana Press Inc.

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Nutikka, A., Binnington-Boyd, B., Lingwood, C.A. (2003). Methods for the Identification of Host Receptors for Shiga Toxin. In: Philpott, D., Ebel, F. (eds) E. coli. Methods in Molecular Medicine™, vol 73. Humana Press. https://doi.org/10.1385/1-59259-316-X:197

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  • DOI: https://doi.org/10.1385/1-59259-316-X:197

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-939-1

  • Online ISBN: 978-1-59259-316-3

  • eBook Packages: Springer Protocols

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