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E. coli pp 187–195Cite as

Methods for the Purification of Shiga Toxin 1

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Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 73))

Abstract

In addition to its involvement in clinical disease, Shiga toxin (Stx) is being appreciated as a useful tool in various aspects of cell physiology. These include defining signal transduction pathways (1–5), intracellular monitors of homeostatic processes (6–8), inducers of apoptosis (9–15), antigen carriers for cellular immunization (16–18), and as an antineoplastic agent (19–24). These various fields of study require the availability of purified toxin and/or its subunits. In order to facilitate the expansion of such studies, in the absence of a commercial supplier, a detailed purification scheme for Stx1 is presented in this chapter.

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Author information

Authors and Affiliations

  1. Departments of Laboratory Medicine and Pathobiology and Biochemistry, University of Toronto and The Research Institute, Hospital for Sick Children, Toronto, Canada

    Anita Nutikka, Beth Binnington-Boyd & Clifford A. Lingwood

Authors
  1. Anita Nutikka
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  2. Beth Binnington-Boyd
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  3. Clifford A. Lingwood
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Editor information

Editors and Affiliations

  1. Groupe d&'Immunité Innée et Signalisation, Institut Pasteur, Paris, France

    Dana Philpott

  2. Max-von-Pettenkofer-Institut, Bakteriologie, Munich, Germany

    Frank Ebel

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© 2003 Humana Press Inc.

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Nutikka, A., Binnington-Boyd, B., Lingwood, C.A. (2003). Methods for the Purification of Shiga Toxin 1. In: Philpott, D., Ebel, F. (eds) E. coli. Methods in Molecular Medicine™, vol 73. Humana Press. https://doi.org/10.1385/1-59259-316-X:187

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  • DOI: https://doi.org/10.1385/1-59259-316-X:187

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-939-1

  • Online ISBN: 978-1-59259-316-3

  • eBook Packages: Springer Protocols

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