Abstract
Retinoblastoma (Rb) is an intraocular tumor usually diagnosed in children under four years of age (1). The tumor rises when both alleles of the Rb tumor suppressor gene become inactivated in a retinal precursor cell during development (2,3). The first retinoblastoma cell line to be established in culture, Y-79 (4), has been shown to originate from neuroectodermal cells that express both neuronal and glial cell markers (3). Both Y-79 cells and Rb tumor cells produce mRNAs encoding several proteins unique to the photoreceptors (5), including different subunits of cone- and rod-specific cGMP-phosphodiesterases (6). Therefore, cultured Y-79 cells, which have a human retinal origin, could be particularly useful for studying the regulatory mechanisms of photoreceptor-specific gene expression (7).
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References
Keith, C. G. (1993) Molecular biology in ocular disorders. Med. J. Australia 158, 615ā618.
Lee, W. H., Bookstein, R., Hong, F., et al. (1987) Human retinoblastoma susceptibility gene: cloning, identification, and sequence. Science 235, 1394ā1399.
Kyritsis, A. P., Tsokos, M., Triche, T. J., and Chader, G. J. (1984) Retinoblastoma-origin from a primitive neuroectodermal cell? Nature 307, 471ā473.
Reid, T. W., Albert, D. M., Rabson, A. S., et al. (1974) Characteristics of an established cell line of retinoblastoma. J. Natl. Cancer Inst. 53, 347ā360.
Di Polo, A. and Farber, D. B. (1995) Rodphotoreceptor-specific gene expression in human retinoblastoma cells. Proc. Natl. Acad. Sci. USA 92, 4016ā4020.
Hurwitz, R. L., Bogenmann, E., Font, R. L., et al. (1990) Expression of the functional cone phototransduction cascade in retinoblastoma. J. Clin. Invest. 85, 1872ā1878.
Di Polo, A., Lerner, L. E., and Farber, D. B. (1997) Transcriptional activation of the human rod cGMP-phosphodiesterase beta-subunit gene is mediated by an upstream AP-1 element. Nucleic Acids Res. 25, 3863ā3867.
Wigler, M., Silverstein, S., Lee, L. S., et al. (1977) Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells. Cell. 11, 223ā232.
Chu, G., Hayakawa, H., and Berg, P. (1987) Electroporation for the efficient transfection of mammalian cells with DNA. Nucleic Acids Res. 15, 1311ā1326.
Felgner, P. L., Gadek, T. R., Holm, M., et al. (1987) Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure. Proc. Natl. Acad. Sci. USA 84, 7413ā7417.
Gorman, C. M., Moffat, L. F., and Howard, B. H. (1982) Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol. Cell Biol. 2, 1044ā1051.
Hall, C. V., Jacob, P. E., Ringold, G. M., and Lee, F. (1983) Expression and regulation of Escherichia coli lacZ gene fusions in mammalian cells. J. Mol. Appl. Genet. 2, 101ā109.
De Wet, J. R., Wood, K. V., DeLuca, M., et al. (1987) Firefly luciferase gene: structure and expression in mammalian cells. Mol. Cell Biol. 7, 725ā737.
Knoll, B. J., Zarucki, S. T., Dean, D. C., and Oā²Malley, B. W. (1983) Definition of the ovalbumin gene promoter by transfer of an ovalglobin fusion gene into cultured cells. Nucleic Acids Res. 11, 6733ā6754.
Sambrook, J., Fritsch, E., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. vol. 3, Cold Spring Harbor Laboratory, New York, pp. 16.66ā16.67.
Lee, S. Y. and Rasheed, S. (1990) A simple procedure for maximum yield of high-quality plasmid DNA. Biotechniques 9, 676ā679.
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Ā© 2001 Humana Press Inc.
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Farber, D.B., Lerner, L.E., Viczian, A.S. (2001). Transient Transfection of Human Retinoblastoma Cells. In: Rakoczy, P.E. (eds) Vision Research Protocols. Methods in Molecular Medicineā¢, vol 47. Humana Press. https://doi.org/10.1385/1-59259-085-3:31
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DOI: https://doi.org/10.1385/1-59259-085-3:31
Publisher Name: Humana Press
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Online ISBN: 978-1-59259-085-8
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