Protocol

Chaperonin Protocols

Volume 140 of the series Methods in Molecular Biology™ pp 41-49

Purification of GroES from an Overproducing E. coliStrain

  • Elsie Quaite-RandallAffiliated withCenter for Mechanistic Biology and Biotechnology, Structure Biology Center, Argonne National Laboratory
  • , Andrzej JoachimiakAffiliated withCenter for Mechanistic Biology and Biotechnology, Structure Biology Center, Argonne National Laboratory

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Abstract

Of the Hsp10/cpn10 family of molecular chaperones, GroES, the cochaperonin or “release factor” from Escherichia coli is the best characterized. Since GroEL and GroES are on the same operon in E. coli (1), they are usually overexpressed together in the same strain (see Note 1). We commonly purify GroES from the same cells used for GroEL purification (2).