Purification of GroES from an Overproducing E. coliStrain

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Of the Hsp10/cpn10 family of molecular chaperones, GroES, the cochaperonin or “release factor” from Escherichia coli is the best characterized. Since GroEL and GroES are on the same operon in E. coli (1), they are usually overexpressed together in the same strain (see Note 1). We commonly purify GroES from the same cells used for GroEL purification (2).