Abstract
The growth of mammalian cells in culture can be monitored by a number of parameters related to the increase of cellular biomass over time. The simplest method is by cell counting at regular intervals. In routine cultures this would be performed once a day, which corresponds to the approximate doubling time of mammalian cells during the exponential growth phase. This would establish an overall growth profile of a culture. More frequent counts would be required to follow more subtle changes that may, for example, be associated with the cell growth cycle.
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© 1999 Humana Press Inc., Totowa, NJ
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Butler, M. (1999). Cell Counting and Viability Measurements. In: Jenkins, N. (eds) Animal Cell Biotechnology. Methods in Biotechnology™, vol 8. Humana Press. https://doi.org/10.1385/0-89603-547-6:131
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DOI: https://doi.org/10.1385/0-89603-547-6:131
Publisher Name: Humana Press
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