Abstract
Ribonucleic acid (RNA) is an unbranched polymer of purine (adenine, guanine) and pyrimidine (cytosine, uracil) nucleotides joined by phosphodiester bonds.
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References
Wyatt J. R. and Tinoco I. J. (1993) RNA structure and RNA function, in The RNA World (Gesteland R. F and Atkins J. F., eds.), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 465–496.
Choi Y. C. and Ro-Choi T.-S. (1980) Basic characteristics of different classes of cellular RNAs a directory, in Gene Expression The Production of RNAs (Goldstein L. and Prescott D. M., eds.), Academic, NY, pp. 609–667
Farrell R. (1993), in RNA Methodologies. A Laboratory Guide for Isolation and Characterization Academic, San Diego, CA.
Gesteland R. F. and Atkins J. F. (1993) The RNA World Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
Williamson R. (1980) The processing of hnRNA and its relation to mRNA, in Gene Expression: The Production of RNAs (Goldstein L. and Prescott D M, eds.), Academic, NY, pp. 547–562
Cohen S. and Stanley W. (1942) The molecular size and shape of the nucleic acid of tobacco mosaic virus. J Biol Chem 144, 589–598
Noll H and Stutz E (1968) The use of sodium and lithium dodecyl sulfate in nucleic acid isolation. Methods Enzymol 12, Part B, 129–155
Glism V., Crkvenjakov R., and Byus C (1974) Ribonucleic acid isolated by cesium chloride centnfugation. Biochemistry 13, 2633–2637.
Kirby K. S. (1968) Isolation of nucleic acids with phenolic solutions. Methods Enzymol 12, part B, 87–99.
Ingle J. and Burns R. G. (1968) The loss of ribosomal ribonucleic acid during the preparation of nucleic acod from certain plant tissues by the detergent-phenol method. Biochem J. 110, 605, 606
Grinnan E. and Mosher W. (1951) Highly polymerized ribonucleic acid: preparation from liver and depolymenzation J. Biol Chem 191, 719–726
Cox R. A. (1968) The use of guanidinium chloride in the isolation of nucleic acids Methods Enzymol 12, Part B, 120–129.
Chirgwin J. M., Przybyka A. E., Mac Donald R. J., Rutter W. J. (1979) Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18, 5294–5299
Chomczynski P. and Sacchi N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162, 156–159.
Macfarlane D E. and Dahle C. E. (1993) Isolating RNA from whole bloods—the dawn of RNA-based diagnosis? Nature 362, 186–188.
Dahle C. E. and Macfarlane D E. (1993) Isolation of RNA from cells in culture using Catrimox-14 cationic surfactant. BioTechniques 15, 1102–1105.
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© 1998 Humana Press Inc.
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Macfarlane, D.E., Dahle, C.E. (1998). Introduction to Isolating RNA. In: Rapley, R., Manning, D.L. (eds) RNA Isolation and Characterization Protocols. Methods in Molecular Biology™, vol 86. Humana Press. https://doi.org/10.1385/0-89603-494-1:1
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DOI: https://doi.org/10.1385/0-89603-494-1:1
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