Abstract
Mycoplasma is the generic term used by cell biologists to denote organisms belonging to the Order Mycoplasmatales, which can infect cell cultures. Of particular interest are those organisms that belong to the Families Mycoplasmataceae (Mycoplasma) and Acholeplasmataceae (Acholeplasma).
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References
Robinson, L. B., Wichelhausen, R. B., and Roizman, B. (1956) Contamination of human cell cultures by pleuropneumonia-like organisms. Science 124, 1147, 1148.
McGarrity, G.]., Phillips, D., and Vaidya, A. (1980) Mycoplasmal infection of lymphocyte cultures: Infection with M. salivarium. In Vitro 16, 346–356.
Butler, M. and Leach, R. H. (1964) A mycoplasma that induces acidity and cytopathic effect in tissue culture. J. Gen. Microbiol. 34, 285–294.
Aula, P. and Nichols, W. W. (1967) The cytogenetic effects of mycoplasma in human leucocyte cutures. J. Cell Physiol. 70, 281–290.
Stanbridge, E. J., Hayflick, L., and Perkins, F. T. (1971) Modification of amino acid concentrations induced by mycoplasmas in cell culture medium. Nature 232, 242–244.
Levine, E. M., Thomas, L., McGregor, D., Hayflick, L. V., and Eagle, H. (1968) Altered nucleic acid metabolism in human cell cultures infected with mycoplasma. Proc. Natl. Acad. Sci. USA 60, 583–589.
Wise, K. S., Cassell, G. H., and Action, R. T. (1978) Selective association of murine T lymphoblastoid cell surface alloantigens with M. hyorhinis. Proc. Natl. Acad. Sci. USA 75, 4479–4483.
MacPherson, I. and Russel, W. (1966) Transformations in hampster cells mediated by mycoplasmas. Nature 210, 1343–1345.
European Pharmacopoeia (1980) Biological Tests. 2nd Ed., Part 1, V. 2.1.3 Mycoplasmas. France: Maisonneuve, S. A.
Code of Federal Regulations (1986) Animals and animal products. 9. Ch. 1. Part 113.28 p. 379. Detection of mycoplasma contamination. Office of the Federal Register National Archives and Records Administration, Washington.
National Collection of Type Cultures, Central Public Health Laboratory, 61, Colindale Avenue, Colindale, London NW9 5 HT.
American Type Culture Collection, 12301 Parklawn Drive, Rockville, Maryland 20852, USA.
Myco-Tect, Bethesda Research Laboratories, Inc., Gaithersburg, Maryland 20877, USA.
Gen-Probe, Inc., 9620 Chesapeake Drive, San Diego, California 92123, USA.
McGarrity, G. J., Kotani, H., and Carson, D. (1986) Comparative studies to determine the efficiency of 6 methylpurine deoxyriboside to detect cell culture mycoplasmas. In Vitro Cellular and Developmental Biology 22, 301–304.
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© 1990 The Humana Press Inc.
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Mowles, J.M. (1990). Mycoplasma Detection. In: Walker, J.M., Pollard, J.W., Walker, J.M. (eds) Animal Cell Culture. Methods in Molecular Biology, vol 5. Humana Press. https://doi.org/10.1385/0-89603-150-0:65
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DOI: https://doi.org/10.1385/0-89603-150-0:65
Publisher Name: Humana Press
Print ISBN: 978-0-89603-150-0
Online ISBN: 978-1-59259-492-4
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