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Quantum Dots

Volume 374 of the series Methods in Molecular Biology pp 147-159

Sentinel Lymph Node Mapping With Type-II Quantum Dots

  • John V. FrangioniAffiliated withDivision of Hematology/Oncology, Department of Radiology, Beth Israel Deaconess Medical Center, Harvard Medical School
  • , Sang-Wook KimAffiliated withDepartment of Chemistry, Massachusetts Institute of Technology
  • , Shunsuke OhnishiAffiliated withDivision of Hematology/Oncology, Department of Radiology, Beth Israel Deaconess Medical Center, Harvard Medical School
  • , Sungjee KimAffiliated withDepartment of Chemistry, Massachusetts Institute of Technology
  • , Moungi G. BawendiAffiliated withDepartment of Chemistry, Massachusetts Institute of Technology

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Abstract

Sentinel lymph node (SLN) mapping is an important cancer surgery during which the first lymph node draining the site of a tumor is identified, resected, and analyzed for the presence or absence of malignant cells. Fluorescent semiconductor nanocrystals (quantum dots [QDs]) of the appropriate size, charge, and emission wavelength permit this surgery to be performed rapidly, with high sensitivity and under complete image guidance. We describe the materials and methods necessary for the production and characterization of type-II near-infrared fluorescent QDs, which have been optimized for SLN mapping. They contain a CdTe core, CdSe shell, and a highly anionic, oligomeric phosphine organic coating. We also describe how to utilize such QDs in animal model systems of SLN mapping.

Key Words

Quantum dots near-infrared fluorescence near-infrared fluorescence imaging imaging intraoperative imaging sentinel lymph nodes