Protocol

Macromolecular Crystallography Protocols

Volume 364 of the series Methods in Molecular Biology™ pp 43-61

First Analysis of Macromolecular Crystals

Biochemistry and X-Ray Diffraction
  • David JeruzalmiAffiliated withDepartment of Molecular and Cellular Biology, Harvard University

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Abstract

Methods are described for performing a first analysis of newly prepared macromolecular crystals. Biochemical analysis using denaturing gel electrophoresis on dissolved crystals informs on the content of the crystal. Detailed protocols are given for mounting crystals in capillaries or fiber loops in preparation for analysis of their X-ray diffractive properties.

Key Words

Macromolecular crystals biochemical analysis of crystal contents crystal mounting X-ray diffraction crystal symmetry space groups