Protocol

Protocols for Nucleic Acid Analysis by Nonradioactive Probes

Volume 353 of the series Methods in Molecular Biology pp 3-13

Genomic DNA Isolation From Different Biological Materials

  • Duckchul ParkAffiliated withEcological Genetics Laboratory, Landcare Research Ltd.

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Abstract

A comprehensive collection of different methods for extracting high-quality genomic DNA from Gram-positive and -negative bacteria and fungal mycelium and spores is described in this chapter. Special care has been taken in describing the ideal ratio of biological material to chemical reagents for an efficient extraction of genomic DNA, and in stating the appropriate application in molecular biology protocols (e.g., PCR or genomic DNA library-cloning quality).

Key Words

Fungal spores fungi mycelium genomic DNA isolation Gram-negative bacteria Gram-positive bacteria