Protocol

Glycobiology Protocols

Volume 347 of the series Methods in Molecular Biology pp 237-252

Micromethods for the Characterization of Lipid A-Core and O-Antigen Lipopolysaccharide

  • Cristina L. MaroldaAffiliated withDepartment of Microbiologoy and Immunology, University of Western Ontario
  • , Piya LahiryAffiliated withDepartment of Microbiologoy and Immunology, University of Western Ontario
  • , Enrique VinésAffiliated withDepartment of Microbiologoy and Immunology, University of Western Ontario
  • , Soledad SaldíasAffiliated withDepartment of Microbiologoy and Immunology, University of Western Ontario
  • , Miguel A. ValvanoAffiliated withDepartment of Microbiologoy and Immunology, University of Western Ontario

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Abstract

Methods for rapid and simple analysis of lipopolysaccharide (LPS) from bacterial whole-cell lysates or membrane preparations have contributed to advancing our knowledge of the genetics of the LPS biogenesis. LPS, a major constituent of the outer membranes in Gram-negative bacteria, has a complex mechanism of synthesis and assembly that requires the coordinated participation of many genes and gene products. This chapter describes a collection of methods routinely used in our laboratory for the characterization of LPS in Escherichia coli and other bacteria.