Protocol

Target Discovery and Validation Reviews and Protocols

Volume 360 of the series Methods in Molecular Biology™ pp 155-161

Production of siRNA and cDNA-Transfected Cell Arrays on Noncoated Chambered Coverglass for High-Content Screening Microscopy in Living Cells

  • Holger ErfleAffiliated withCell Biology and Cell Biophysics Unit, European Molecular Biology Laboratory
  • , Rainer PepperkokAffiliated withCell Biology and Cell Biophysics Unit, European Molecular Biology Laboratory

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Summary

In this chapter, we provide a protocol for the production of transfected cell arrays in living mammalian cells on noncoated chambered coverglass for the systematic functional analyses of human genes by high-content screening microscopy. This method should facilitate drug target validation by small-interfering RNAs.

Key Words

Cell arrays genomics high-content screening microscopy small-interfering RNAs siRNA