Protocol

Agrobacterium Protocols Volume 2

Volume 344 of the series Methods in Molecular Biology pp 273-285

Grapevine (Vitis vinifera L.)

  • Alain BouquetAffiliated withUMR Biology of Development of Cultivated Perennial Plants, Agro-M/INRA
  • , Laurent TorregrosaAffiliated withUMR Biology of Development of Cultivated Perennial Plants, Agro-M/INRA
  • , Pat IoccoAffiliated withCSIRO Plant Industry, Horticulture Unit
  • , Mark R. ThomasAffiliated withCSIRO Plant Industry, Horticulture Unit

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Abstract

Grapevine (Vitis) is considered to be one of the major fruit crops in the world based on hectares cultivated and economic value. Grapes are used not only for wine but also for fresh fruit, dried fruit, and juice production. Wine is by far the major product of grapes and the focus of this chapter is on wine grape cultivars. Grapevine cultivars of Vitis vinifera L. have a reputation for producing premium quality wines. These premium quality wines are produced from a small number of cultivars that enjoy a high level of consumer acceptance and are firmly entrenched in the market place because of varietal name branding and the association of certain wine styles and regions with specific cultivars. In light of this situation, grapevine improvement by a transgenic approach is attractive when compared to a classical breeding approach. The transfer of individual traits as single genes with a minimum disruption to the original genome would leave the traditional characteristics of the cultivar intact. However, a reliable transformation system is required for a successful transgenic approach to grapevine improvement. There are three criteria for achieving an efficient Agrobacterium-mediated transformation system: (1) the production of highly regenerative transformable tissue, (2) optimal co-cultivation conditions for both grapevine tissue and Agrobacterium, and (3) an efficient selection regime for transgenic plant regeneration. In this chapter, we describe a grapevine transformation system which meets the above mentioned criteria.

Key Words

Grapevine Vitis embryogenic callus Agrobacterium selectable markers transgenic antibiotic sensitivity reporter genes plant regeneration transformation efficiency