Protocol

MicroRNA Protocols

Volume 342 of the series Methods in Molecular Biology™ pp 129-138

miRBase:The MicroRNA Sequence Database

  • Sam Griffiths-JonesAffiliated withWellcome Trust Sanger Institute, Wellcome Trust Genome Campus

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Abstract

The miRBase Sequence database is the primary repository for published microRNA (miRNA) sequence and annotation data. miRBase provides a user-friendly web interface for miRNA data, allowing the user to search using key words or sequences, trace links to the primary literature referencing the miRNA discoveries, analyze genomic coordinates and context, and mine relationships between miRNA sequences. miRBase also provides a confidential gene-naming service, assigning official miRNA names to novel genes before their publication. The methods outlined in this chapter describe these functions. miRBase is freely available to all at http://​microrna.​sanger.​ac.​uk/​.

Key Words

MicroRNA homolog gene names noncoding RNA