Protocol

Placenta and Trophoblast

Volume 122 of the series Methods in Molecular Medicine™ pp 225-239

In Vitro Models for Studying Trophoblast Transcellular Transport

  • Claudia J. BodeAffiliated withDepartment of Pharmaceutical Chemistry, School of Pharmacy, University of Kansas
  • , Hong JinAffiliated withDepartment of Pharmaceutical Chemistry, School of Pharmacy, University of Kansas
  • , Erik RyttingAffiliated withDepartment of Pharmaceutical Chemistry, School of Pharmacy, University of Kansas
  • , Peter S. SilversteinAffiliated withDepartment of Pharmaceutical Chemistry, School of Pharmacy, University of Kansas
  • , Amber M. YoungAffiliated withDepartment of Pharmaceutical Chemistry, School of Pharmacy, University of Kansas
  • , Kenneth L. AudusAffiliated withDepartment of Pharmaceutical Chemistry, School of Pharmacy, University of Kansas

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Abstract

In vitro models have proven to be effective in studying the placental transporters that play a role in the exchange of nutrients, waste products, and drugs between the maternal and fetal circulations. Although primary cultures of trophoblast cells can be used to perform uptake, efflux, and metabolism studies, only the rodent HRP-1 and the human BeWo cell lines have been shown to form confluent monolayers when grown on semi-permeable membranes. Protocols for the revival, maintenance, passage, and growth of BeWo cells for transporter expression and transcellular transport studies are provided.

Key Words

Trophoblast cells BeWo cell transcellular transport efflux mechanisms