Peptide Synthesis and Applications

Volume 298 of the series Methods in Molecular Biology™ pp 227-240

Protein Identification by Mass Spectrometric Analyses of Peptides

  • Ashley MartinAffiliated withCancer Research UK Institute for Cancer Studies, University of Birmingham

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The focus of this chapter is the isolation and analysis of subsets of proteins or subproteomes. These methodologies can be applied to address fundamental questions in many areas of biological research. Protocols are provided for common techniques that include protein isolation from mammalian cells and the subsequent identification of peptides derived from endogenous proteins by the action of proteases.

Key Words

Mass spectrometry proteome SDS-PAGE trypsin digest cell lysis