Protocol

Human Cell Culture Protocols

Volume 107 of the series Methods in Molecular Medicine™ pp 183-206

Well-Differentiated Human Airway Epithelial Cell Cultures

  • M. Leslie FulcherAffiliated withCystic Fibrosis/Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill
  • , Sherif GabrielAffiliated withCystic Fibrosis/Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill
  • , Kimberlie A. BurnsAffiliated withCystic Fibrosis/Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill
  • , James R. YankaskasAffiliated withCystic Fibrosis/Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill
  • , Scott H. RandellAffiliated withCystic Fibrosis/Pulmonary Research and Treatment Center, The University of North Carolina at Chapel Hill

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Abstract

The airway epithelium occupies a critical environmental interface, protecting the host from a wide variety of inhaled insults, including chemical and particulate pollutants and pathogens. The coordinated regulation of ion and water transport, mucous secretion, and cilia beating underlies mucociliary clearance. Physical trapping and removal of harmful substances, in combination with baseline or inducible secretion of antimicrobial factors, antioxidants, and protease inhibitors and recruitment of nonspecific inflammatory cells (neutrophils, monocytes), constitutes airway innate host defense.