DNA Viruses

Volume 292 of the series Methods in Molecular Biology pp 267-276

DNA Affinity Purification of Epstein-Barr Virus OriP-Binding Proteins

  • Constandache AtanasiuAffiliated withThe Wistar Institute
  • , Larissa LezinaAffiliated withThe Wistar Institute
  • , Paul M. LiebermanAffiliated withThe Wistar Institute

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DNA affinity purification has been used to identify cellular and viral proteins associated with the Epstein-Barr virus origin of plasmid DNA replication. This approach allows for a one- or two-step purification scheme of high-affinity DNA binding proteins from crude nuclear extracts. Additionally, this approach may be useful for isolation of proteins that are found in the insoluble fractions of the nuclear matrix or scaffold.

Key Words

DNA affinity magnetic beads OriP EBNA-1 telomere repeat binding factors