Determining Protein Half-Lives
- Pengbo Zhou
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Controlling the stability of cellular proteins is a fundamental way by which cells regulate growth, differentiation, survival, and development. Measuring the turnover rate of a protein is often the first step in assessing whether or not the function of a protein is regulated by proteolysis under specific physiological conditions. Over the years, procedures to determine the half-life of proteins in cultured eukaryotic cells have been well-established. This chapter describes in detail the two most frequently used methods, pulse-chase analysis and cycloheximide blocking, to determine a protein’s half-life in yeast and cultured mammalian cells.
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- Determining Protein Half-Lives
- Book Title
- Signal Transduction Protocols
- pp 67-77
- Print ISBN
- Online ISBN
- Series Title
- Methods in Molecular Biology
- Series Volume
- Series ISSN
- Humana Press
- Copyright Holder
- Humana Press
- Additional Links
- Protein degradation
- Industry Sectors
- Editor Affiliations
- 1. Department of Molecular and Cellular Biochemistry, College of Medicine, University of Kentucky
- Pengbo Zhou (2)
- Author Affiliations
- 2. Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, New York, NY
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