Ubiquitination of G-Protein-Coupled Receptors
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In this chapter we describe methods for detecting the ubiquitination state of G-protein-coupled receptors (GPCRs). This involves coexpression of a GPCR with an epitope-tagged ubiquitin construct in a heterologous expression system. Modification by ubiquitin of the GPCR resulting from agonist activation is detected by immunoprecipation and subsequent immunoblotting for the epitope-tagged ubiquitin. We use here the chemokine receptor CXCR4 as the model receptor; however, this could be easily modified to detect the ubiquitination state of any GPCR.
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- Ubiquitination of G-Protein-Coupled Receptors
- Book Title
- Receptor Signal Transduction Protocols
- pp 299-305
- Print ISBN
- Online ISBN
- Series Title
- Methods in Molecular Biology
- Series Volume
- Series ISSN
- Humana Press
- Copyright Holder
- Humana Press
- Additional Links
- G-protein-coupled receptor
- Industry Sectors
- Editor Affiliations
- 1. Department of Cell Physiology and Pharmacology, University of Leicester
- Author Affiliations
- 2. Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA
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