Bacterial Artificial Chromosomes

Volume 255 of the series Methods in Molecular Biology™ pp 279-294

Using the TIGR Assembler in Shotgun Sequencing Projects

  • Mihai PopAffiliated withThe Institute for Genomic Research
  • , Dan KosackAffiliated withThe Institute for Genomic Research

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The TIGR Assembler (TA) (1) is the sequence assembly program used in sequencing projects at The Institute for Genomic Research (TIGR). Development of the TA was based on the experience obtained in more than 20 sequencing projects completed at TIGR (see http://​www.​tigr.​org). This extensive experience led to a sequence assembler that produces few misassemblies (2,3) and has been used successfully in whole-genome shotgun sequencing of prokaryotic and eukaryotic organisms, bacterial artificial chromosome-based sequencing of eukaryotic organisms, and expressed sequence tag assembly.