Bacterial Artificial Chromosomes

Volume 255 of the series Methods in Molecular Biology™ pp 47-56

Construction of Small Genome BAC Library for Functional and Genomic Applications

  • David ŠmajsAffiliated withDepartment of Microbiology and Molecular Genetics, University of Texas-Houston Medical School
  • , Steven J. NorrisAffiliated withDepartment of Pathology and Laboratory Medicine, University of Texas-Houston Medical School
  • , George M. WeinstockAffiliated withHuman Genome Sequencing Center, Baylor College of Medicine

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The use of genetic approaches to study bacteria is limited by the following considerations: first, the bacteria must be easy to culture and nonpathogenic; and, second, a broad spectrum of genetic techniques for the particular bacteria being studied must be available. For other bacteria, such as the syphilis-causing spirochete, Treponema pallidum, genetic studies are highly limited because the bacterium cannot be continuously grown in the laboratory, because of its high virulence and a total lack of genetic tools to study this organism.