Protocol

Cytokine Protocols

Volume 249 of the series Methods in Molecular Biology pp 201-218

Detection of Cytokine- and Chemokine-Expressing Cells at the Single Cell Level

  • Karin LoréAffiliated withCenter for Infectious Medicine, Department of Medicine, Karolinska Institutet, Huddinge University Hospital
  • , Jan AnderssonAffiliated withCenter for Infectious Medicine, Department of Medicine, Karolinska Institutet, Huddinge University Hospital

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Methods for detection of cytokines and chemokines at a protein level in individual cells based on immunofluorescence or immunohistochemical (116) staining techniques have been developed in our laboratory. The difficulties in these procedures lie in the critical steps of fixation and permeabilization of the cells in combination with a very careful selection of suitable cytokine- or chemokine-specific antibodies.