Antibody Engineering

Volume 248 of the series Methods in Molecular Biology™ pp 431-441

Characterization of Antibody-Antigen Interactions by Fluorescence Spectroscopy

  • Sotiris MissailidisAffiliated withDepartment of Chemistry, The Open University
  • , Kevin BradyAffiliated withSchool of Chemistry, University of Nottingham

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Fluorescence spectroscopy is a widely used technique for the characterization of molecular interactions in biological systems. It is highly sensitive, and allows measurements at low sample concentrations (down to 10-7 M). It is also available at a reasonably low equipment cost. These features make fluorescence spectroscopy an attractive technique as compared to other forms of optical spectroscopy.