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G Protein Signaling

Volume 237 of the series Methods in Molecular Biology™ pp 21-38

Purification of G Protein Subunits from Sf9 Insect Cells Using Hexahistidine-Tagged α and βγ Subunits

  • Tohru KozasaAffiliated withDepartment of Pharmacology, University of Illinois at Chicago

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Summary

G protein-mediated pathways are the most fundamental mechanisms of cell signaling. In order to analyze these pathways, the availability of purified recombinant G proteins are critically important. Using Sf9-Baculovirus expression system, a general and simplified method to purify various G protein subunits is described in this chapter. This method is useful for purification of most of G protein subunits.

Key Words

G protein recombinant protein Sf9-Baculovirus expression system