PCR Protocols

Volume 226 of the series Methods in Molecular Biology™ pp 33-34

DNA Extraction from Tissue

  • Helen PearsonAffiliated withMolecular Endocrinology Unit, Western General Hospital
  • , David StirlingAffiliated withDepartment of Hematology, Royal Infirmary of Edinburgh

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The following protocol is one of the longest-established methods of DNA extraction and works well with a wide range of solid tissues. Proteins are digested with proteinase K and extracted with phenol chloroform. DNA is then precipitated with ethanol. The resultant DNA (10–50 μg) is of high molecular weight and is a suitable template for long polymerase chain reaction (PCR).