Nanoelectrospray Tandem Mass Spectrometry and Sequence Similarity Searching for Identification of Proteins from Organisms with Unknown Genomes

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Recent developments in technology and instrumentation have made mass spectrometry the method of choice for identification of proteins in a sequence database [reviewed in refs. (14)].Regardless of what mass spectrometric technology is applied, it is ultimately required that acquired mass spectra be accurately matched to a protein sequence from the corresponding database entry.