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Tumor Suppressor Genes

Volume 223 of the series Methods in Molecular Biology™ pp 73-86

Genetic Strategies in Saccharomyces cerevisiae to Study Human Tumor Suppressor Genes

  • Takahiko KobayashiAffiliated withHokkaido University Medical Hospital
  • , Ting WangAffiliated withDepartment of Genetics, Washington University School of Medicine
  • , Hua QianAffiliated withDepartment of Surgery, Shanghai Second Medical University
  • , Rainer K. BrachmannAffiliated withDepartment of Medicine, University of California at IrvineDepartment of Biological Chemistry, University of California at Irvine

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Abstract

Baker’s yeast or Saccharomyces cerevisiae is the most intensely studied eukaryotic microorganism for very good reasons. Many fundamental processes such as cell cycle control and DNA repair show evolutionary conservation from yeast to human, thus making studies in yeast highly relevant to our understanding of mammalian cells. Yeast also allows for the efficient pursuit of questions that are often almost impossible to answer in mammalian assay systems.