PCR Mutation Detection Protocols

Volume 187 of the series Methods in Molecular Biology pp 73-86

Fluorescent In Situ Hybridization

  • Sara A. DyerAffiliated withRegional Genetics Laboratory, Birmingham Women’s Hospital
  • , Elaine K. GreenAffiliated withDepartment of Psychiatry, Queen Elizabeth Psychiatric Hospital, University of Birmingham

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Single-stranded DNA will recognize a complementary strand with high specificity under suitably controlled conditions. In situ hybridization (ISH) exploits this phenomenon by hybridizing an appropriately labeled singlestranded DNA “probe” to target sequences in situ in either dissociated cell preparations or tissue sections.