Serum-Free Media for Neural Cell Cultures
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The ability to grow primary neurons under serum-free conditions is facilitating better control in studies of neuronal development, mechanisms of neuronal signaling, electrophysiology, pharmacology, plasticity, in vitro growth requirements, gene expression, and neurotoxicity. Some of the new commercially available media combinations allow for the growth of sparse populations of neurons, which in turn allow for the study of individual neurons and synapses. This has not been possible using serum-supplemented media without a feeder layer of glial cells. In serum-supplemented media, glial cells continue to multiply, necessitating the use of cytotoxic mitotic inhibitors (Wallace and Johnson, 1989). Serum also contains unknown and variable levels of growth factors, hormones, vitamins, and proteins.
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- Serum-Free Media for Neural Cell Cultures
- Book Title
- Protocols for Neural Cell Culture
- pp 255-264
- Print ISBN
- Online ISBN
- Humana Press
- Copyright Holder
- Humana Press
- Additional Links
- Editor Affiliations
- 1. Department of Anatomy and Cell Biology, School of Medicine, University of Saskatchewan
- Author Affiliations
- 2. Life Technologies, Inc., Grand Island, NY
- 3. School of Medicine, Southern Illinois University, Springfield, IL
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