Protocol

Bioinformatics Methods and Protocols

Volume 132 of the series Methods in Molecular Biology™ pp 365-386

Primer3 on the WWW for General Users and for Biologist Programmers

  • Steve RozenAffiliated withWhitehead Institute for Biomedical Research
  • , Helen SkaletskyAffiliated withWhitehead Institute for Biomedical Research

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Abstract

Designing PCR and sequencing primers are essential activities for molecular biologists around the world. This chapter assumes acquaintance with the principles and practice of PCR, as outlined in, for example, refs. 14.