Protein Structure Calculation from NMR Data

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Until 1984, structural information of biomolecules at atomic resolution could only be determined by X-ray diffraction techniques with protein single crystals (1). In the mid-1980s, Wüthrich and co-workers demonstrated that nuclear magnetic resonance (NMR) spectroscopy (2) could be used as a technique for protein structure determination (3). This permits biomolecular structure determination