Protocol

Calcium-Binding Protein Protocols: Volume 2: Methods and Techniques

Volume 173 of the series Methods in Molecular Biology™ pp 267-283

Protein Structure Calculation from NMR Data

  • Tapas K. MalAffiliated withDepartment of Medical Biophysics, Ontario Cancer Institute, University of Toronto
  • , Stefan BagbyAffiliated withDepartment of Medical Biophysics, Ontario Cancer Institute, University of Toronto
  • , Mitsuhiko IkuraAffiliated withDivision of Medical and Structural Biology, Department of Medical Biophysics, Ontario Cancer Institute, University of Toronto

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Abstract

Until 1984, structural information of biomolecules at atomic resolution could only be determined by X-ray diffraction techniques with protein single crystals (1). In the mid-1980s, Wüthrich and co-workers demonstrated that nuclear magnetic resonance (NMR) spectroscopy (2) could be used as a technique for protein structure determination (3). This permits biomolecular structure determination