Transgenesis Techniques

Volume 180 of the series Methods in Molecular Biology pp 305-341

Analysis of Transgenic Mice

  • Stefan SelbertAffiliated withMice and More GmbH and Co.
  • , Dominic RannieAffiliated withDepartment of Pathology, University of Edinburgh Medical School

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The first step in the analysis of potentially transgenic mice is the characterizaton of their altered genotype. A strategy must be devised that allows easy and reliable discrimination between wild-type animals and those carrying additional transgenes or introduced targeted mutations. It is also necessary to demonstrate that the characteristics of the alteration are as desired.