Protocol

Cytochrome P450 Protocols

Volume 107 of the series Methods in Molecular Biology™ pp 295-302

Isolation of Rat Hepatocytes

  • Karen De SmetAffiliated withDepartment of Toxicology, Vrije Universiteit Brussel
  • , Sonja BekenAffiliated withDepartment of Toxicology, Vrije Universiteit Brussel
  • , Tamara VanhaeckeAffiliated withDepartment of Toxicology, Vrije Universiteit Brussel
  • , Marleen PauwelsAffiliated withDepartment of Toxicology, Vrije Universiteit Brussel
  • , Antoine VercruysseAffiliated withDepartment of Toxicology, Vrije Universiteit Brussel
  • , Vera RogiersAffiliated withDepartment of Toxicology, Vrije Universiteit Brussel

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Abstract

Because the liver is the main organ involved in the metabolism and toxicity of xenobiotics, isolated hepatocytes from various species, mcludmg humans, and their cultures, constitute attractive in vitro models for pharmaco-toxtcological studies (see Chapter 30).