Quantitative PCR Protocols

Volume 26 of the series Methods in Molecular Medicine™ pp 277-287

A Stochastic PCR Approach for RNA Quantification in Multiple Samples

  • Adrian PuntschartAffiliated withDepartment of Anatomy, University of Bern
  • , Michael VogtAffiliated withDepartment of Anatomy, University of Bern

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When studying the effect of various treatments on gene expression in humans, one occasionally is faced with the problem of detecting small changes in transcript levels in minute tissue samples. In addition, interindividual variations can be quite large and may even be the major source of variation (1). Therefore, numerous samples usually have to be analyzed to detect such small variations in gene expression.