Protocol

Mycobacteria Protocols

Volume 101 of the series Methods in Molecular Biology™ pp 431-455

The Use of Luciferase-Reporter Phage for Antibiotic-Susceptibility Testing of Mycobacteria

  • Paul F. RiskaAffiliated withDivision of Infectious Diseases, Albert Einstein College of Medicine
  • , William R. JacobsJr.Affiliated withDivision of Infectious Diseases, Albert Einstein College of Medicine

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Prior to this decade, routine antibiotic-susceptibility testing (AST) of Mycobacterium tuberculosis clinical isolates was not advocated (1) This policy was predicated on the following assumptions:
  1. 1.

    The prevalence ofantibiotic resistance is low, occurring primarily among patients previously treated for tuberculosis (TB) who have relapsed,

     
  2. 2.

    Tuberculous infection progresses slowly, so that resistant strains of M tuberctlosis can be treated empirically with sequential therapeutic regimens without incurring great risks; and

     
  3. 3

    The long delay before AST information is available (6–12 wk) precludes a practical impact on patient care.