Protocol

Basic Cell Culture Protocols

Volume 75 of the series Methods in Molecular Biology™ pp 131-144

Tissue Culture of Skeletal Muscle

  • Terry A. PartridgeAffiliated withMRC Clinical Sciences Centre, Royal Postgraduate Medical School, Hammersmith Hospital

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Abstract

In its simple forms, no special difficulty attaches to the tissue culture of skeletal muscle. Indeed, it is one of the easiest tissues to culture in large amounts because the starting material, skeletal muscle, is plentiful and readily obtainable from a wide variety of species, including humans. Moreover, the stem cells from which muscle develops in tissue culture seem to be very resilient; they will survive anoxic conditions within muscle at temperatures between 4 and 37°C, for up to a few days, and when obtained in suspension, are easy to freeze (with 10% dimethyl sulfoxide [DMSO] as a cryoprotectant) and store in liquid nitrogen.