Establishment and Maintenance of Cell Suspension Cultures
Purchase on Springer.com
$49.95 / €39.95 / £34.95*
* Final gross prices may vary according to local VAT.
Cell suspension cultures are rapidly dividing homogenous suspensions of cells grown in ltqutd nutrient media from which samples can be precisely removed (1). Cell suspensions are used for generating large amounts of cells for quantitative or qualitative analysis of growth responses and metabolism of novel chemicals, as well as for studies of cell cycle under standard conditions (2,3). In addition, cell suspensions serve as an ideal material for the isolation of protoplasts used in transient gene expression assays and Agrobacterzum— mediated transformation (4,5). The establishment of suspension cultures of Arabidopsis thalzana cells derived from leaf and hypocotyl calli has been reported (6). In order to mitiate Arabzdopsis suspensions retammg a high regenerative potential, a procedure described by Ford (7) has been modified Callus tissues derived from root or hypocotyl explants of Arabzdopszs yield well proliferating cell suspensions capable of morphogenesis m liquid medium. The regeneration capability and genetic stability of suspension cells, however, decrease by the length of culture period Therefore, it is recommended to use newly initiated cell suspensions when the applications require the regeneration of dtplotd fertile plants The method described is used to establish morphogenic cell suspensions from the Arabidopszs ecotypes Columbia, C24, RLD, and Wassilewskila. Slight modification of the concentration of growth regulators (e.g., auxm) and/or the time of subcultures may be required for other ecotypes.
- King, P J. (1984) Induction and maintenance of cell suspension cultures, in Cell Culture and Somatrc Cell Genetm of Plants vol 0 (Vasil, I K ed) Academic, New York, pp 130–138
- May, M J and Leaver, C. J (1993) Oxtdattve sttmulatton of glutathtone synthesis of Arabldopsls thallana suspension cultures Plant Physzol 103, 621–627.
- Magyar, Z., Bakó, L, Bogre, L, Dedeoglu, D., Kapros, T., and Dudtts, D (1993) Acttvecdc2 genes and cell cycle phase spectfic cdc2–related kmase complexes m hormoneósttmulated alfalfa cells. Plant J 4, 151–161 CrossRef
- Doellmg, J. H. and Ptkaard, C. S. (1993) Transient expression mArabldopsrs thalrana protoplasts derived from rapidly established cell suspension cultures Plant Cell Rep 12, 241–244.
- An, G (1985) Hugh efftctency transformatton of cultured tobacco cells Plant Physrol 79,568–570 CrossRef
- Gleddie, S. (1989) Plant regeneration from cell suspension cultures ofArabzdopm thalrana Heynh Plant Cell Rep. 8, 1–5 CrossRef
- Ford, K G. (1990) Plant regeneratton from Arabzdopszs thalzana protoplasts Plant Cell Rep 8,534–537. CrossRef
- Murashige, T. and Skoog, F (1962) A revised medmm for rapid growth and bioassays with tobacco tissue cultures Phystol Plant 15, 473–497. CrossRef
- Gamborg, 0. L, Miller, R A and Ojtma, K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50, 151–158 CrossRef
- Establishment and Maintenance of Cell Suspension Cultures
- Book Title
- Arabidopsis Protocols
- Book Part
- pp 27-30
- Print ISBN
- Online ISBN
- Series Title
- Methods in Molecular Biology™
- Series Volume
- Series ISSN
- Humana Press
- Copyright Holder
- Humana Press
- Additional Links
- Industry Sectors
- Editor Affiliations
- 1. Centro de Investigación y Tecnologia, Instituto Nacional de Investigación y Tecnologia Agraria y Alimentaria
- Author Affiliations
- 2. Max, Planck-Institut fur Zuchtungsforschung, Cologne, Germany
To view the rest of this content please follow the download PDF link above.