Protocol

Arabidopsis Protocols

Volume 82 of the series Methods in Molecular Biology™ pp 259-266

In Planta AgrobacteriumMediated Transformation of Adult Arabidopsis thaliana Plants by Vacuum Infiltration

  • Nicole BechtoldAffiliated withLaboratoire de GOnOtique et AmOlioration des Plantes, INRA-Centre de Versailles
  • , Georges PelletierAffiliated withLaboratoire de GOnOtique et AmOlioration des Plantes, INRA-Centre de Versailles

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Abstruct

Plant genetic transformation was initiated and developed m the 80s thanks to the convergence of constant progress m the protocol of regeneratton from tissue culture, molecular techniques leading to well-expressed marker genes after transfer m plant cells, and the dtverslficatlon of DNA delivery methods.