Electrotransformation of the Spirochete Borrelia burgdorferi
- D. Scott Samuels
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Borrelia burgdorferi is an etiologic agent of Lyme disease, the most common arthropod-borne disease in the United States (1,2). The bacterium, a member of the spirochete phylum, has a genome predominantly composed of linear DNA molecules (3,4). Formulating a medium in which B. burgdorferi grows in vitro was the first step toward a genetic understanding of the physiology and pathogenesis of the organism (5,6). The growth of B. burgdorferi as single colonies in solid medium (7–9) has facilitated mutant isolation by selection (10,11), although a defined medium for selection of auxotrophs is not currently available. The transformation system described in this chapter will be useful for manipulating the spirochete on a molecular genetic level.
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About this Protocol
- Electrotransformation of the Spirochete Borrelia burgdorferi
- Book Title
- Electroporation Protocols for Microorganisms
- pp 253-259
- Print ISBN
- Online ISBN
- Series Title
- Methods in Molecular Biology™
- Series Volume
- Series ISSN
- Humana Press
- Copyright Holder
- Humana Press
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- Jac A. Nickoloff (1)
- Editor Affiliations
- 1. Department of Cancer Biology, Harvard University School of Public Health
- D. Scott Samuels (2)
- Author Affiliations
- 2. Bacterial Pathogenesis Section, Rocky Mountain Laboratories Microscopy Branch, National Institute of Allergy and Infectious Diseases, Hamilton, MT
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