Basic Protein and Peptide Protocols

Volume 32 of the series Methods in Molecular Biology™ pp 433-440

Enzyme and Biotin Labeling of Antibody

  • G. Brian WisdomAffiliated withDivision of Biochemistry, The Queen’s University, Medical Biology Centre

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Labeled antibodies are key reagents in many procedures, particularly immunoassays and the detection of antigens in tissue sections and on membrane blots. The ideal labeled antibody retains all the immunoreactivity of the antibody and all the signal-generating capacity of the label. In addition, these conjugates should be stable, and have defined and appropriate proportions of the two components.