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Continuous-Flow Electrophoresis

Application to the Isolation of Lysosomes and Endosomes

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Biomembrane Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 19))

Abstract

Continuous-flow electrophoresis (CFE), which separates particles on the basis of surface charge density, should be regarded as an adjunct to centrifugation rather than as an alternative for membrane fractionation. The equipment is expensive and has been used for relatively specialized tasks, not for the routine isolations from a total homogenate.

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References

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© 1993 Humana Press Inc. Totowa, NJ

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Graham, J.M. (1993). Continuous-Flow Electrophoresis. In: Graham, J.M., Higgins, J.A. (eds) Biomembrane Protocols. Methods in Molecular Biology, vol 19. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-236-1:41

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  • DOI: https://doi.org/10.1385/0-89603-236-1:41

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-236-1

  • Online ISBN: 978-1-59259-506-8

  • eBook Packages: Springer Protocols

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