Immunochemical Protocols

Volume 10 of the series Methods in Molecular Biology pp 105-116

Epitope Mapping

  • Sara E. MoleAffiliated withCRC Human Cancer Genetics Research Group, Department of Pathology, University of Cambridge

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Monoclonal antibodies (MAbs) are specific immunological tools because they bind to a precise determinant (the epitope) on the surface of a protein. The procedure of identifying the binding site of a MAb is often termed “epitope mapping.”