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Fluorescent In Situ Hybridization

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PCR Mutation Detection Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 187))

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Abstract

Single-stranded DNA will recognize a complementary strand with high specificity under suitably controlled conditions. In situ hybridization (ISH) exploits this phenomenon by hybridizing an appropriately labeled singlestranded DNA “probe” to target sequences in situ in either dissociated cell preparations or tissue sections.

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References

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Author information

Authors and Affiliations

  1. Regional Genetics Laboratory, Birmingham Women’s Hospital, Birmingham, UK

    Sara A. Dyer

  2. Department of Psychiatry, Queen Elizabeth Psychiatric Hospital, University of Birmingham, Birmingham, UK

    Elaine K. Green

Authors
  1. Sara A. Dyer
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  2. Elaine K. Green
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Editor information

Editors and Affiliations

  1. Department of Haematology, Birmingham Children’s Hospital NHS Trust, Birmingham, UK

    Bimal D. M. Theophilus

  2. Department of Biosciences, University of Hertfordshire, Hatfield, UK

    Ralph Rapley

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© 2002 Humana Press Inc., Totowa, NJ

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Dyer, S.A., Green, E.K. (2002). Fluorescent In Situ Hybridization. In: Theophilus, B.D.M., Rapley, R. (eds) PCR Mutation Detection Protocols. Methods in Molecular Biology, vol 187. Humana Press. https://doi.org/10.1385/1-59259-273-2:073

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  • DOI: https://doi.org/10.1385/1-59259-273-2:073

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-617-8

  • Online ISBN: 978-1-59259-273-9

  • eBook Packages: Springer Protocols

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