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M13 Cloning Vehicles

Their Contribution to DNA Sequencing

  • Protocol
DNA Sequencing Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 23))

Abstract

For studies in molecular biology, DNA purification has been essential, in particular for DNA sequencing, probing, and mutagenesis. The amplification of DNA in E. coli by cloning vehicles derived from M13mp or pUC made expensive physical separation techniques like ultracentrifugation unnecessary. Although today the polymerase chain reaction is a valuable alternative for the amplification of small DNA pieces (1), it cannot substitute for the construction of libraries of DNA fragments. Therefore, E. coli has served not only as a vehicle to amplify DNA, but also to separate many DNA molecules of similar length and the two DNA strands simultaneously. For this purpose, a bacteriophage like M 13 can be used. The various viral cis- and trunsacting functions are critical not only for strand separation, but also to separate the single-stranded DNA from the E. coli cell by an active transport mechanism through the intact cell wall.

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References

  1. Saiki, R. K., Gelfand, D. H., Stoffel, S, Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., and Ehrlich, H A. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239, 487–491

    Article  PubMed  CAS  Google Scholar 

  2. Scott, J. and Smith, G.(1990) Searchmg for peptIde ligands with an epitope library. Science 249, 386–390.

    Article  PubMed  CAS  Google Scholar 

  3. Messing, J., Crea, R., and Seeburg, P. H. (1981) A system for shotgun DNA sequencing. Nucl. Acids Res. 9, 309–321.

    Article  PubMed  CAS  Google Scholar 

  4. Norrander, J., Kempe, T., and Messing, J (1983) Improved M13 vectors using oligonucleotide-directed mutagenesis. Gene 26, 101–106.

    Article  PubMed  CAS  Google Scholar 

  5. Edgell, M. H., Hutchison, C. A., III, and Sclair, M. (1972) Specific endonuclease R fragments of bacteriophage $X174 deoxyribonucleic acid. J. Viral. 9, 574–582

    CAS  Google Scholar 

  6. Beaucage, S. L. and Caruthers, M H. (1981) Deoxynucleoside phosphoramidites-a new class of key Intermediates for deoxypolynucleotide synthesis. Tetrahedron Lett. 22, 1859–1862.

    Article  CAS  Google Scholar 

  7. Hutchison, C. A., III, Phtlhps, S., Edgell, M. H., Gillam, S., Jahnke, P., and Smith, M (1978) Mutagenesis at a specific position in a DNA sequence. J. Biol. Chem. 253, 6551–6560.

    PubMed  CAS  Google Scholar 

  8. Smith, M, Leung, D W, Gillam, S., Astell, C. R, Montgomery, D. L., and Hall, B D (1979) Sequence of the gene for iso-1-cytochrome C in Saccharomyces cerevisiae. Cell 16, 753–761.

    Article  PubMed  CAS  Google Scholar 

  9. Zoller, M and Smith, M. (1982) Oligonucleotide-directed mutagenesis using M13-derived vectors an efficient and general procedure for the production of point mutations in any fragment of DNA. Nucl. Acids Res. 10, 6487–6500

    Article  PubMed  CAS  Google Scholar 

  10. Vieira, J. and Messing, J(1982) The pUC plasmtds, an M 13mp7 derived system for msertion mutagenesis and sequencing with synthetic universal prtmers Gene 19, 259–268.

    Article  PubMed  CAS  Google Scholar 

  11. Sanger, F, Donelson, J E., Coulson, A. R., Kossel, H., and Fischer, H (1973) Use of DNA polymerase I primed by a synthetic ohgonucleotide to determine a nucleotide sequence in phage f 1 DNA. Proc. Nat1 Acad. Ser. USA 70, 1209–1213

    Article  CAS  Google Scholar 

  12. Hofschneider, P H (1963) Untersuchungen uber “kleine” E coli K12 Bacteriophagen M12, M13, und M20. Z. Naturforschg. 18b, 203–205.

    CAS  Google Scholar 

  13. Davison, A. J. (1991) Experience in shotgun sequencing a 134 kilobase pair DNA molecule DNA Sequence 1, 389–394

    PubMed  CAS  Google Scholar 

  14. Bolivar, F, Rodriguez, R. L., Greene, P. J., Betlach, M. V., Heynecker, H L, Boyer, H. W, Crosa, J. W, and Flakow, S (1977) Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2, 95–113

    Article  PubMed  CAS  Google Scholar 

  15. Herrmann, R., Neugebauer, K., Zentgraf, H., and Schaller, H. (1978) Transposition of a DNA sequence determining kanamycin resistance into the singlestranded genome of bacteriophage fd Mol Gen. Genet. 159, 171.

    Article  PubMed  CAS  Google Scholar 

  16. Vovis, G F. and Ohsumi, M. (1978) The filamentous phages as transducing particles, in The Single-Stranded DNA Phages (Denhardt, D. T, Dressler, D., and Ray, D S, eds ), Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp 445–448

    Google Scholar 

  17. Salivar, W. O., Henry, T. J., and Pratt, D. (1967) Purtfication and properties of diploid particles of coliphage M13. Virology, 32, 41–51

    Article  PubMed  CAS  Google Scholar 

  18. Mandel, M. and Higa, A (1970) Calcium-dependent bacteriophage DNA mfection. J. Mol Blol., 53, 159–162.

    Article  CAS  Google Scholar 

  19. Malamy, M H., Fiandt, M, and Szybalski, W. (1972) Electron microscopy of polar insertions in the lac operon of Escherichia coli Mol. Gen Genet 119, 207–222

    Article  PubMed  CAS  Google Scholar 

  20. Landy, A, Olchowski, E, and Ross, W. (1974) Isolation of a functional lac regulatory region. Mol. Gen Genet 133, 273–281.

    Article  PubMed  CAS  Google Scholar 

  21. Messing, J. (1991) Clonmg in M13 phage or how to use biology at its best Gene 100, 3–12

    Article  PubMed  CAS  Google Scholar 

  22. Messing, J, Gronenborn, B., Muller-Hill, B., and Hofschnetder, P H. (1977) Frlamentous cohphage M13 as a cloning vehtcle insertion of a HindII fragment of the lac regulatory region in the Ml3 rephcattve form in vitro Proc Natl. Acad. Sci. USA 74, 3642–3646.

    Article  PubMed  CAS  Google Scholar 

  23. Messing, J. and Gronenborn, B (1978) The filamentous phage M13 as carrter DNA for operon fusions in vitro, in The Single-Stranded DNA Phages (Denhardt, D T., Dressler, D., and Ray, D. S., eds.), Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp. 449–453.

    Google Scholar 

  24. Gronenborn, B. and Messing, J. (1978) Methylatron of single-stranded DNA m vttro mtroduces new restrtctrons endonuclease cleavage sites. Nature 272, 375–377

    Article  PubMed  CAS  Google Scholar 

  25. Dotto, G. P. and Zmder, N D (1984) Reduction of the minimal sequence for mmatton of DNA synthesis by qualitative and quantttatrve changes of an mltrator protein Nature 311, 279–280

    Article  PubMed  CAS  Google Scholar 

  26. Heidecker, G., Messmg, J, and Gronenborn, B. (1980) A versatile prrmer for DNA sequencing m the M13mp2 clonmg system Gene 10, 69–73.

    Article  PubMed  CAS  Google Scholar 

  27. Messing, J (1979) A multrpurpose cloning system based on the single-stranded DNA bacteriophage M13. Recombinant DNA Technical Bulletin, NIH Publtcanon No. 79-99,2, No. 2, 43–48

    CAS  Google Scholar 

  28. Gardner, R. C., Howarth, A J., Hahn, P O, Brown-Leudi, M, Shepherd, R J., and Messmg, J. (1981) The complete nucleotide sequence of an mfectious clone of cauliflower mosaic virus by M13mp7 shotgun sequencmg. Nucl Acrds Res. 9, 2871–2888

    Article  CAS  Google Scholar 

  29. Holden, C(1991) Briefings Science 254, 28

    Google Scholar 

  30. Messing, J (1983) New M13 vectors for clonmg. Methods Enzymol. 101, 20–78

    Article  PubMed  CAS  Google Scholar 

  31. Larson, R. and Messing, J. (1982) Apple II software for M13 shotgun DNA sequencmg Nucl Acids Res 10, 39–49

    Article  PubMed  CAS  Google Scholar 

  32. Larson, R. and Messing, J. (1983) Apple II computer software for DNA and protean sequence data DNA 2, 31–35.

    PubMed  CAS  Google Scholar 

  33. Hackett, P. H., Fuchs, J A, and Messing, J (1984) An introduction to recombmant DNA techniques Basic Experiments in Gene Manzpulution Benjamin-Cummings, Menlo Park, CA

    Google Scholar 

  34. Messing, J., Vieira, J, and Gardner, R (1982) Codon insertion mutagenesis to study functional domains of β-lactamase In vrtro mutagenesis Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp 52

    Google Scholar 

  35. Messmg, J. and Seeburg, P H (1981) A strategy for hrgh speed DNA sequencing, in Developmental Biology Using Purified Genes (Brown, D. and Fox, F, eds ), ICN-UCLA Symposia on Molecular and Cellular Biology, vol 23 Academic, NY, pp 659–669.

    Google Scholar 

  36. Yanisch-Perron, C, Vieira, J, and Messing, J (1985) Improved M13 phage cloning vectors and host strains, nucleottde sequences of the M 13mp and pUC vectors Gene 33, 103–119

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Waksman Institute, Piscataway, NJ

    Joachim Messing

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  1. Joachim Messing
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Editor information

Editors and Affiliations

  1. Institute of Food Research, Norwich Research Park, Norwich, England

    Hugh G. Griffin  & Annette M. Griffin  & 

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© 1993 Humana Press Inc. Totowa, New Jersey

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Messing, J. (1993). M13 Cloning Vehicles. In: Griffin, H.G., Griffin, A.M. (eds) DNA Sequencing Protocols. Methods in Molecular Biology™, vol 23. Humana Press. https://doi.org/10.1385/0-89603-248-5:9

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  • DOI: https://doi.org/10.1385/0-89603-248-5:9

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-248-4

  • Online ISBN: 978-1-59259-510-5

  • eBook Packages: Springer Protocols

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