Abstract
The comet assay was developed as a sensitive method for detecting DNA damage and repair at the level of individual cells. The combination of comet assay with fluorescence in situ hybridization (FISH) or comet-FISH technique permits to localize DNA breaks within the specific DNA sequences. The position of FISH signals within the comet head or tail indicates intact or damaged DNA in genome region selected by the probe, respectively. Comet-FISH offers unique possibility to detect on the same specimen the total DNA damage and evaluate the damage of specific regions of genome as well.
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Hovhannisyan, G., Aroutiounian, R. (2017). Comet-FISH. In: Liehr, T. (eds) Fluorescence In Situ Hybridization (FISH). Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-52959-1_39
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DOI: https://doi.org/10.1007/978-3-662-52959-1_39
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