Abstract
Insight into the dynamics of RNA biosynthesis, processing, and cellular activity is highly valuable because it will deepen our understanding of cell physiology and help explain how mRNA-misregulation contributes to the development of many diseases. To date, the study of mRNA inside cells is a challenge; many analytical approaches focus only on quantifying expression levels of transcripts and are not capable of reporting their intracellular locations, which have emerged as a critical determinant of RNA function. Similarly, techniques capable of probing RNA localization merely offer snapshots in fixed cells. Herein, we describe a protocol based on the bacteriophage MS2 genetic system for use in detecting mRNA in living cells.
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Ma, B., Tanese, N. (2017). RNA Imaging in Living Cells. In: Liehr, T. (eds) Fluorescence In Situ Hybridization (FISH). Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-52959-1_15
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DOI: https://doi.org/10.1007/978-3-662-52959-1_15
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Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-662-52957-7
Online ISBN: 978-3-662-52959-1
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