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Analyzing Microbes pp 169–186Cite as

ELISA-Based Identification and Detection of Microbes

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Abstract

Enzyme-linked immunosorbent assay (ELISA) is an analytical technique to detect the presence of an antigen or antibody in a given sample. It shows wider applications in clinical diagnosis, in pathological studies, and in quality control studies. Virtually all microbial species have unique antigen(s), and such type of antigen(s) can be exploited as specific molecules of detection by ELISA. The variations in ELISA allow us to detect either antigen or antibody, identifying the different strains of microbes at a time and also in characterization of the epitope distribution on the microbial surface. Five types of variants have been developed in this assay: (1) Direct ELISA-use in the detection of antigen; (2) Indirect ELISA-use in the detection of antibody; (3) Sandwich ELISA-use in identification of different epitopes at a time; (4) Competitive ELISA-use in quantifying the antigen/antibody, and (5) Multiplex ELISA-use in identification of multiple antigens/antibodies at a time. Here, we discuss about different variants of ELISA and add detailed steps in performing these ELISA methods with their advantages and limitations.

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Author information

Authors and Affiliations

  1. Department of Biotechnology, School of Bioscience and Biotechnology, Babasaheb Bhimrao Ambedkar University, Lucknow, Uttar Pradesh, India

    Jyoti Verma, Sangeeta Saxena & Sunil G. Babu

Authors
  1. Jyoti Verma
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  2. Sangeeta Saxena
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  3. Sunil G. Babu
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Editor information

Editors and Affiliations

  1. National Bureau of Agriculturally Import, Kusumaur, Maunath Bhanjan, 275101, Uttar Pradesh, India

    Dilip Kumar Arora

  2. , Dep. Life Sciences, National Institute of Technology, Rourkela, Orissa, India

    Surajit Das

  3. National Bureau of Agriculturally Import, Kusumaur, Maunath Bhanjan, 275 101, Uttar Pradesh, India

    Mesapogu Sukumar

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© 2013 Springer-Verlag Berlin Heidelberg

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Verma, J., Saxena, S., Babu, S.G. (2013). ELISA-Based Identification and Detection of Microbes. In: Arora, D., Das, S., Sukumar, M. (eds) Analyzing Microbes. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-34410-7_13

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  • DOI: https://doi.org/10.1007/978-3-642-34410-7_13

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-34409-1

  • Online ISBN: 978-3-642-34410-7

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