Aside from microscopically visible chromosomal rearrangements, numerous cryptic chromosomal alterations have been reported since the introduction of techniques like FISH, array-CGH or MLPA. This is especially true of dynamic regions in the subtelomere and subcentromere of any chromosome. To address these regions, locus-specific FISH probes are employed as single, chromosome-specific or genome-wide probe sets. Here we present the chromosome-specific subtelomere–subcentromere multicolor FISH (subCTM) and genome-wide subcentromere multicolor FISH (subcenM) probe sets, which are useful as screening tools in specific patient groups like the infertile and mentally retarded, as well as in tumor cytogenetics and for evolutionary studies when applied as ZOO-FISH.
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Acknowledgments
Supported in parts by the Dr. Robert Pfleger-Stiftung, DFG (436 RUS 17/135/03, 17/109/04 and 17/22/06, 436 WER 17/1/04 and 17/5/05, WE 3617/2-1), DAAD/ British Council support (313-ARC-XX-lk, A0703172/Ref.325), Boehringer Ingelheim Fonds, Stefan-Morsch-Stiftung, Erwin-Riesch Stiftung, and Evangelische Studienwerk e.V. Villigst.
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Weise, A., Mrasek, K., Gross, M., Klaschka, V., Liehr, T. (2009). Subtelomeric and/or Subcentromeric Probe Sets. In: Liehr, T. (eds) Fluorescence In Situ Hybridization (FISH) — Application Guide. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-70581-9_24
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DOI: https://doi.org/10.1007/978-3-540-70581-9_24
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